Coding

Part:BBa_K3264007:Design

Designed by: Xinyou Chang   Group: iGEM19_GreatBay_SZ   (2019-10-16)


NTWCT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 460
    Illegal AgeI site found at 961
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the linker between the NT/CT and the W(repetitive region) is GNS The construct was cloned into a pT7 plasmid and transformed into BL21 (DE3) E. coli.



Source

de novo synthesize DNA from Recombinant protein, Domain from Euprosthenops australis 、Araneus ventricosus and Argiope trifasciata

References